FIG. 1.

Evaluation of mouse microbead-based KIM-1 assay. (A) Mouse recombinant KIM-1 protein was serially diluted and 13-point standard curve was generated by microbead-based ELISA assay. (B) Precision profile for KIM-1 assay. The intra- and interassay reproducibility of the assay was assessed by measuring 13 serially diluted samples (50,000 to 12.21 pg/ml of KIM-1). For the intra-assay variability, each sample was measured in six replicates within one plate. For the interassay variability, each sample was measured 5 times in five different plates. (C) Linearity of dilution of KIM-1 assay. Urine samples were diluted (1:2, 1:4, 1:8, 1:10, 1:16, 1:32, and 1:64) in sample diluents, and KIM-1 levels were evaluated in triplicate. The percent recovery is calculated.