FIG. 9.

Detection of KIM-1 using mouse Rena-Stick in AA model. KIM-1 in standard solution or urine samples is measured by mixing 60 μl of sample with 60 μl of TRIS-buffered solution and adding the mix to the sample port in Rena-Stick. Results were read in 15min. (A) A visual increase in KIM-1 band intensity at various time points after one dose of AA (5mg/kg). KIM-1 values obtained by quantification of band intensity using the lateral-flow reader were plotted versus values obtained by microbead-based assay in urines collected from AA-treated mice (B) and control mice (C).