TABLE 1 .
Performance Characteristics of Microbead KIM-1 Assay and Rena-Stick Assay
| Assay parameters | Microbead-based assay | Rena-Stick assay |
|---|---|---|
| Assay range | 12.21 pg/ml to 50ng/ml | 195 pg/ml to 50ng/ml |
| Sensitivity | Lowest limit of detection12.21 pg/ml | Lowest limit of detection190 pg/ml |
| Dilution linearity | Linear across dilutions 1:8, 1:10, 1:16, and 1:20 | Linear across dilutions 1:8, 1:10, 1:16, and 1:20 |
| Spike recovery | 90–110% | 80–85% |
| Cross-reactivity/specificity | No cross-reactivity with human and rat KIM-1 | No cross-reactivity with human and rat KIM-1 |
| Intra-assay variability | 0.73–5.41% | 8.2–14.5% |
| Interassay variability | 3.73–10.15% | 12.4–16.3% |
| Volume requirement of specimen to measure in triplicate | 10 μl | 180 μl |
| Time for the assay | ~2 h | 15 min |
| High-throughput | Yes | No |