FIG. 4.

Low-dose arsenic in combination with ethanol activates PI3K/Akt signaling in colon cancer cells. (A) DLD-1 cells were exposed to arsenic or ethanol either alone or combined at the indicated concentrations in a time course, after which Western blot assays were carried out using anti-PI3K (Y458), anti-PI3K, anti-Akt (Y308), anti-Akt (S473), anti-Akt, anti-GSK3β (S9), anti-GSK3β and anti-GAPDH antibodies. (B) Densitometry analysis of expression level from Western blot as in (A). (C) DLD-1 cells were treated as described in (A) for 24h. Western blot assays were performed. (D). Densitometry analysis of expression level from Western blot as in (C).

Columns, mean of densitometry of band vs. loading control from three independent experiments.