FIG. 6.

Low-dose arsenic in combination with ethanol enhances tumor angiogenesis. (A) Arsenic and/or ethanol exposed DLD-1 medium induced HUVEC migration in Boyden chamber assay. (B) Cells migrated through the membrane as shown in (A) were quantified by manual counting. (C) HUVEC migration in wound healing migration assay. (D) Migrated HUVEC shown in (C) were quantified by manual counting. (E) Medium from DLD-1 cells treated with arsenic and/or ethanol induced tube formation of HUVEC. (F) Branch points in (E) were quantified by manual counting. (G) Catalase or SOD suppresses arsenic and/or ethanol-induced tube formation of HUVEC. (H). Branch points in (G) were quantified by manual counting. (I) MnTBAP or HIF-1 inhibitor LW6 suppresses arsenic and/or ethanol-induced tube formation of HUVEC. (J) Branch points in (I) were quantified by manual counting. (K) In vivo Matrigel plug assays using medium from DLD-1 cells treated with arsenic and/or ethanol.

Columns, mean of three duplicates; bars, SE (*p ≤ 0.05).