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Quantitative real-time PCR revealed: (A) No significant differences among three age groups in the levels of the mRNA that encodes type-I collagen; (B)(C) The level of the mRNA that encodes MMP-2 and MMP-9 increased with age (*p < 0.001, n = 3); (D)(E) The level of the mRNA that encodes TIMP-1 and TIMP-2 decreased with age (*p < 0.001, n = 3); (F) No significant differences among three age groups in the level of the mRNA that encodes TGF-β1.
