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(A) Zymography of conditioned medium revealed the enzymatic activities of MMP-2 (lower band: 72 kDa) and MMP-9 (upper band: 92 kDa). (B)(C) Densitometric analysis of MMP-2 and −9, with levels normalized to the number of viable cells determined using the MTT assay, revealed markedly higher MMP-2 and −9 activity in senescent tenocytes than in the young tenocytes (*p < 0.001, n = 3).
