Table 1.

Primer sequences of target genes for real-time PCR

Target genes	Primer sequence
GAPDH	5’ AGTCTACTGGCGTCTTCA 3’ forward
	5’ TTGTCATATTTCTCGTGGT 3’ reverse
COL1A1	5’ TACAGCACGCTTGTGGATG 3’ forward
	5’ TTGGGATGGAGGGAGTTTA 3’ reverse
MMP-2	5’ GGAAGCATCAAATCGGACTG 3’ forward
	5’ GGGCGGGAGAAAGTAGCA 3’ reverse
MMP-9	5’ CCCACTTACTTTGGAAACG 3’ forward
	5’ GAAGATGAATGGAAATACGC 3’ reverse
TIMP-1	5’ GCCTCTGGCATCCTCTTG 3’ forward
	5’ CTGCGGTTCTGGGACTTG 3’ reverse
TIMP-2	5’ CCAAAGCAGTGAGCGAGAA 3’ forward
	5’ CCCAG GGCAC AATAA AGTC 3’ reverse
TGF-beta-1	5’ AGAGATTCAAGTCAAACTGTGGAG 3’ forward
	5’ CCAAGGTAACGCCAGGAA 3’ reverse

Abbreviation: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; COL, collagen; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; TGF, transforming growth factor.