Figure 3. CYP gene expression at different developmental stages and phylogenetic analysis coexpressed with LSS.

(a) Two-way clustering of gene-expression profiles for the CYP genes expressed across three developmental stages: mycelia (M), primordial (P) and fruiting bodies (F), as quantified by real-time PCR. The relative expression level of each gene was centred on the mean and then unit scaled across the developmental stages. The floor (shown in green) and ceiling (shown in red) of the expression levels were set as twice the s.d. (b) The phylogenetic analysis of CYPs coexpressed with LSS in G. lucidum (GL) and their homologues in Polyporales. A total of 78 coexpressed CYPs from 21 families in G. lucidum and CYPs from the same families in P. placenta (PP) and P. chrysosporium (PC) were included in the tree. The minimal evolution tree was generated with a heuristic search using the Close-Neighbour-Interchange (CNI) algorithm in MEGA (version 5.05). Bootstrap values based on 1,000 replications was set and shown between 50 and 100 just as the branch colours changed from blue, black to red. Moreover, the genes from the same family or subfamily were collapsed and shown as triangles.