Fig. 4.

Screen for mutations of TaABA8′OH1. Using the S2A2 primer pair, the M2 population of gamma-ray treated ‘Tamaizumi’ was screened to detect a deletion mutation in TaABA8′OH. (A) PCR fragments amplified by the S2A2 primer pair. One mutant in TaABA8′OH1-A, TM1833, did not amplify the fragment derived from the A genome. (B) PCR fragments amplified by the dS1dA1 primer pair. The length of the fragment derived from the D genome in mutants (‘Shinchunaga’, ‘Tamaizumi’ and its mutant TM1833) was 966 bp longer than that in the normal type (‘Norin 61’).