Fig. 7.

Changes in seed growth, embryonic ABA content and expression levels of TaABA8′OHs in embryos during development of ‘Tamaizumi’ and TM1833 seeds. Seeds were collected from field-grown wheat at 5-day intervals. Seeds were used without artificial drying and storage. (A) Changes in seed weight and water content of seeds during seed development. Seed weight of ‘Tamaizumi’ (black circle) and TM1833 (white circle) seeds 5 days after imbibition and water content of ‘Tamaizumi’ (filled triangle) and TM1833 (open triangle) seeds are shown. (B) Changes in embryonic ABA contents during grain development. Endogenous ABA content in embryos from ‘Tamaizumi’ (black circle) and TM1833 (white circle) seeds is shown. (C, D) Changes in expression levels of TaABA8′OHs in embryos during seed development. The expression levels of TaABA8′OH1 (C) and TaABA8′OH2 (D) were analyzed by QRT-PCR. Results were normalized to the expression of 18S ribosomal RNA (internal control). Results from three independent experiments are shown with error bars (SE).