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. 2013 Apr 15;140(8):1645–1654. doi: 10.1242/dev.088013

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 2. — Adult puf-8(-/-); gap-3(-/-) germline contain only mitotic germ cells. (A) Distribution pattern of GAP-3::GFP fusion protein expressed in the germline using the upstream and downstream sequences of pie-1. The fusion protein localizes predominantly to the cell membrane of germ cells. (B) Dissected, DAPI-stained gonads. Although the puf-8(-/+); gap-3(-/-) heterozygous germline contains oocytes in the proximal region, the germline homozygous for both mutations does not have oocytes. The broken lines in the left image mark the boundaries among the indicated stages. Only mitotic cells were observed in the double mutant gonad shown on the right. (C) Dissected gonads immunostained for the mitosis marker phosphohistone H3 (PH3). Unlike the puf-8(-/+); gap-3(-/-) heterozygous germline, which contains PH3-positive cells only in the distal part, the double mutant has PH3-positive cells throughout the germline. In addition, the number of PH-3-positive cells in the distal region in the double mutant is significantly more than the single mutant.