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. 2013 Apr 15;140(8):1693–1702. doi: 10.1242/dev.089656

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 4. — Dsh is the target of Cul-3-Dbo/Kel. (A-D) dsh^V26 FRT19A/FRT19A; ptc-GAL4/+; Ubc12^IR-7375R-3, Ubx-FLP/+ (A), dsh^V26 FRT19A/Ubx-FLP FRT19A; ptc-GAL4/Cul-3^IR-109415 (B), dsh^V26 FRT19A/Ubx-FLP FRT19A; kel^DE1 FRT40/arm-lacZ FRT40 (C) and dsh^V26 FRT19A/Ubx-FLP FRT19A; ptc-GAL4/+; faf^IR-2956/+ (D). Wings are stained for Stbm (green), Dsh (red) and β-gal (blue, C); yellow bars mark the ptc-GAL4 domain. Compare Stbm levels in the same wing in the presence or absence of dsh activity. (A,B) Stbm no longer accumulates in dsh clones. (C) In dsh; kel double-mutant tissue (arrow), Stbm no longer accumulates compared with kel mutant tissue (loss of blue), and levels are similar to those upon loss of dsh alone (arrowhead). (D) The dsh clone crosses the ptc-GAL4 boundary, and where faf^IR is expressed (above the arrow) Stbm levels are decreased. Scale bar: 20 μm. (E-G) Western blots showing pulldown of Myc-tagged Dbo and Kelch by GFP-tagged Dsh in S2 cells (E,G) and COS-7 cells (F). Loading of input is 1% of pulldown. Molecular mass (kDa) is shown to the left.