Fig. 7.

Ectopic canal rim epithelium in cβcat^Δexon3 mouse mutants occurs in the absence of prosensory development. (A-F) The expression of Fgf10, Sox2 and Jag1 was downregulated in the anterior (arrows in B,D,F) and posterior prosensory domains of cβcat^Δexon3 mutants at E12.5 (tamoxifen administered at E9.5). The lateral prosensory domain was unaffected. (G-J) Hair (myosin VIIa) and support (Sox2) cell markers were reduced in cβcat^Δexon3 mutants compared with controls at E14.5. (K) Quantification of hair and support cells in control and cβcat^Δexon3 embryos. Error bars indicate s.e.m. ^***P<0.01, ^**P<0.05 (Student’s t-test).