Fig. 6.

Afadin is not required for proximal-distal patterning in nephron precursors. Localization of segment-specific proteins in E14.5 kidneys from controls (Afadin^fl/fl) and mutants (Afadin^fl/fl; Pax3-cre) are indicated. NCAM delineates the renal vesicle (RV) and S-shaped body (SB). Distal (D), mid- (M) and proximal (P) regions are marked. (A-B′) In RVs, WT1 (green) is proximal whereas Lef1 (red) is distal in both control and mutant. (C-D′) In SBs, WT1 (green) is proximal, whereas Lef1 (red) is mid-SB and distal SB in control and mutant. (E-H′) Epha4 (green) is produced in the distal RV and mid-SB in control and mutant. (I-J′) Jag1 (red) localizes to mid-SB in control and mutant. Results are representative of sections from at least two mice. Scale bars: 10 μm.