Figure 3. PAS2 expression in the epidermis is essential for plant development.
(A) Quantification of PAS2 expression levels in 5-d-old seedlings. The mRNA levels were normalized to TUBULIN4. The expression level in wild-type expressing ProATML1:PAS2RNAi is indicated as a relative value, with that in wild-type set to 1. Data are presented as mean ± SD (n = 3). (B) 5-d-old seedlings of wild-type and wild-type expressing ProATML1:PAS2RNAi with a severe phenotype. Transverse sections of shoot apices of 7-d-old seedlings are shown in (C). (D) 5-d-old seedlings of pas2-1 and pas2-1 expressing ProATML1:PAS2–GUS. (E) Transverse section of the shoot apex of a 5-d-old pas2-1 seedling expressing ProATML1:PAS2–GUS. Bars, 1 mm (B, D), 200 µm (C), and 100 µm (E).