Table 1.
Name | Location at genomea | mfeb | Countc | locationd | Sequencee |
---|---|---|---|---|---|
Known miRNA | |||||
sja-bantamf |
SJC_S000254:283545:283639:+ |
−27.5 |
3488 |
3p |
TGAGATCGCGATTAAAGCTGG |
sja-let-7 |
SJC_S005824:19533:19622:+ |
−33.1 |
50 |
5p |
GGAGGTAGTTCGTTGTGTGGT |
sja-miR-2a |
SJC_S000054:242652:242727:- |
−34.2 |
4406 |
3p |
TCACAGCCAGTATTGATGAAC |
sja-miR-2b |
SJC_S000054:242557:242635:- |
−33.6 |
500 |
3p |
TATCACAGCCCTGCTTGGGACAC |
sja-miR-2c |
SJC_S000102:360645:360725:+ |
−28.42 |
62 |
3p |
TATCACAGCCGTGCTTAAGGGC |
sja-miR-2d |
SJC_S000102:360534:360627:+ |
−33.1 |
727 |
3p |
TATCACAGTCCTGCTTAGGTGACG |
sja-miR-2e |
SJC_S000054:242457:242536:- |
−24.7 |
1721 |
3p |
TATCACAGTCCAAGCTTTGG |
sja-miR-71 |
SJC_S000054:242738:242817:- |
−33.01 |
19048 |
5p |
TGAAAGACGATGGTAGTGAGA |
sja-miR-71b |
SJC_S000102:360305:360397:+ |
−36.2 |
144949 |
5p |
TGAAAGACTTGAGTAGTGAGACGC |
sja-miR-124 |
SJC_S000254:113694:113791:+ |
−29.8 |
32 |
3p |
TAAGGCACGCGGTGAATGTCA |
sja-miR-8 |
SJC_S001790:88898:88974:+ |
−31.5 |
12 |
3p |
TAATACTGTTAGGTAAAGATGC |
sja-miR-2162 |
SJC_S000471:21744:21822:- |
−37.9 |
13 |
3p |
TATTATGCAACGTTTCACTCT |
sja-miR-10g |
SJC_S000052:310019:310093:+ |
−23.4 |
2698/11 |
5p/3p |
AACCCTGTAGACCCGAGTTTG/AAATTCGAGTCTATAAGGA |
Novel miRNA | |||||
Epa-miR-01 |
SJC_S000054:242558:242634:- |
−31.1 |
97 |
3p |
TATCACAGCCCTGCTTGGGACA |
Epa-miR-02 |
SJC_S000057:543062:543147:- |
−20.4 |
7 |
5p |
AGAAGGCTGCGTGTTCGGATC |
Epa-miR-03 |
SJC_S000065:176156:176237:+ |
−22 |
7 |
5p |
CTGTCTTCTCTCTCATGTTC |
Epa-miR-04 |
SJC_S000083:92590:92686:+ |
−27.7 |
16 |
3p |
GTTTGAGACTCCGAATGATG |
Epa-miR-05 |
SJC_S000102:360305:360397:+ |
−36.2 |
151 |
5p |
TGAAAGACTTGAGTAGTGAG |
Epa-miR-06 |
SJC_S000102:360535:360626:+ |
−33.1 |
111 |
3p |
TATCACAGTCCTGCTTAGGTGAC |
Epa-miR-07 |
SJC_S000102:360645:360725:+ |
−28.42 |
62 |
3p |
TATCACAGCCGTGCTTAAGGGC |
Epa-miR-08 |
SJC_S000115:155214:155289:- |
−18.2 |
138 |
3p |
GCTATCTCGTCGATACTGGC |
Epa-miR-09 |
SJC_S000254:113694:113791:+ |
−29.8 |
32 |
3p |
TAAGGCACGCGGTGAATGTCA |
Epa-miR-10 |
SJC_S000254:283542:283641:+ |
−30.7 |
176 |
3p |
TGAGATCGCGATTAAAGCTGGTT |
Epa-miR-11 |
SJC_S000471:21744:21822:- |
−37.9 |
13 |
3p |
TATTATGCAACGTTTCACTCT |
Epa-miR-12 |
SJC_S001790:88898:88974:+ |
−31.5 |
12 |
3p |
TAATACTGTTAGGTAAAGATGC |
Epa-miR-13 |
SJC_S005824:19533:19622:+ |
−33.1 |
50 |
5p |
GGAGGTAGTTCGTTGTGTGGT |
Epa-miR-14g | SJC_S008424:1:71:+ | −30.1 | 33/8 | 5p/3p | GACGGGGTGGCCGAGTGGTT/CCATTGGGGTTTCCCCGCGT |
Note: a location of mature miRNAs at the reference genome of Schistosoma japonicum; b energy of stem-loop structure of miRNA precursors, with unit as Kcal/mol; c sequencing count; d location of a mature miRNA at the 3p or 5p arm of its precursor; e sequence of mature miRNA; f Known miRNAs matched perfectly with that from S. japonicum deposited in the miRBase database; g the only two miRNAs (miR-10, Epa-miR-14) that had mature miRNAs both detected at the 5p and 3p arms of their precursors in the E. pancreaticum. Blue: the largest family members of miR-2.