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. 2013 Jan 25;6:25. doi: 10.1186/1756-3305-6-25

Table 1.

Known and novel microRNAs (miRNAs) identified from the pancreatic fluke Eurytrema pancreaticum

Name Location at genomea mfeb Countc locationd Sequencee
Known miRNA
sja-bantamf
SJC_S000254:283545:283639:+
−27.5
3488
3p
TGAGATCGCGATTAAAGCTGG
sja-let-7
SJC_S005824:19533:19622:+
−33.1
50
5p
GGAGGTAGTTCGTTGTGTGGT
sja-miR-2a
SJC_S000054:242652:242727:-
−34.2
4406
3p
TCACAGCCAGTATTGATGAAC
sja-miR-2b
SJC_S000054:242557:242635:-
−33.6
500
3p
TATCACAGCCCTGCTTGGGACAC
sja-miR-2c
SJC_S000102:360645:360725:+
−28.42
62
3p
TATCACAGCCGTGCTTAAGGGC
sja-miR-2d
SJC_S000102:360534:360627:+
−33.1
727
3p
TATCACAGTCCTGCTTAGGTGACG
sja-miR-2e
SJC_S000054:242457:242536:-
−24.7
1721
3p
TATCACAGTCCAAGCTTTGG
sja-miR-71
SJC_S000054:242738:242817:-
−33.01
19048
5p
TGAAAGACGATGGTAGTGAGA
sja-miR-71b
SJC_S000102:360305:360397:+
−36.2
144949
5p
TGAAAGACTTGAGTAGTGAGACGC
sja-miR-124
SJC_S000254:113694:113791:+
−29.8
32
3p
TAAGGCACGCGGTGAATGTCA
sja-miR-8
SJC_S001790:88898:88974:+
−31.5
12
3p
TAATACTGTTAGGTAAAGATGC
sja-miR-2162
SJC_S000471:21744:21822:-
−37.9
13
3p
TATTATGCAACGTTTCACTCT
sja-miR-10g
SJC_S000052:310019:310093:+
−23.4
2698/11
5p/3p
AACCCTGTAGACCCGAGTTTG/AAATTCGAGTCTATAAGGA
Novel miRNA
Epa-miR-01
SJC_S000054:242558:242634:-
−31.1
97
3p
TATCACAGCCCTGCTTGGGACA
Epa-miR-02
SJC_S000057:543062:543147:-
−20.4
7
5p
AGAAGGCTGCGTGTTCGGATC
Epa-miR-03
SJC_S000065:176156:176237:+
−22
7
5p
CTGTCTTCTCTCTCATGTTC
Epa-miR-04
SJC_S000083:92590:92686:+
−27.7
16
3p
GTTTGAGACTCCGAATGATG
Epa-miR-05
SJC_S000102:360305:360397:+
−36.2
151
5p
TGAAAGACTTGAGTAGTGAG
Epa-miR-06
SJC_S000102:360535:360626:+
−33.1
111
3p
TATCACAGTCCTGCTTAGGTGAC
Epa-miR-07
SJC_S000102:360645:360725:+
−28.42
62
3p
TATCACAGCCGTGCTTAAGGGC
Epa-miR-08
SJC_S000115:155214:155289:-
−18.2
138
3p
GCTATCTCGTCGATACTGGC
Epa-miR-09
SJC_S000254:113694:113791:+
−29.8
32
3p
TAAGGCACGCGGTGAATGTCA
Epa-miR-10
SJC_S000254:283542:283641:+
−30.7
176
3p
TGAGATCGCGATTAAAGCTGGTT
Epa-miR-11
SJC_S000471:21744:21822:-
−37.9
13
3p
TATTATGCAACGTTTCACTCT
Epa-miR-12
SJC_S001790:88898:88974:+
−31.5
12
3p
TAATACTGTTAGGTAAAGATGC
Epa-miR-13
SJC_S005824:19533:19622:+
−33.1
50
5p
GGAGGTAGTTCGTTGTGTGGT
Epa-miR-14g SJC_S008424:1:71:+ −30.1 33/8 5p/3p GACGGGGTGGCCGAGTGGTT/CCATTGGGGTTTCCCCGCGT

Note: a location of mature miRNAs at the reference genome of Schistosoma japonicum; b energy of stem-loop structure of miRNA precursors, with unit as Kcal/mol; c sequencing count; d location of a mature miRNA at the 3p or 5p arm of its precursor; e sequence of mature miRNA; f Known miRNAs matched perfectly with that from S. japonicum deposited in the miRBase database; g the only two miRNAs (miR-10, Epa-miR-14) that had mature miRNAs both detected at the 5p and 3p arms of their precursors in the E. pancreaticum. Blue: the largest family members of miR-2.