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. Author manuscript; available in PMC: 2013 Apr 9.
Published in final edited form as: Oncogene. 2009 Dec 14;29(8):1179–1189. doi: 10.1038/onc.2009.404

Figure 5.

Figure 5

MTA1 is needed for HBx stimulated NF-κB signalling. (A) Effect of selective knockdown of MTA1 on the activation status of the NF-κB signaling components in HepG2 cells by Western blot analysis after being transfected with either control vector or HBx (250 ng per reaction in a 6-well plate). HepG2 cells transfected with increased amount of control vector and HBx (50 ng, 250 ng, and 600 ng per reaction) were used as controls. (B) NF-κB-promoter activity in HepG2 cells with or without MTA1 knockdown by siRNA-MTA1 after being transfected with either vector or HBx. Lower panel is the control Western blot analysis for the aforementioned experiments. Vinculin was used as a control. (C) NF-κB-promoter activity in MEF cells after being transfected with either vector or HBx. Lower panel is the control Western blot analysis for the aforementioned experiments. Vinculin was used as a control. (D) Nucleus extracts from HepG2 cells transfected with either vector control or HBx expression vector after MTA1 knockdown by siRNA-MTA1 were subjected to EMSA analysis using a NF-κB-consensus sequence. Extracts from wild type HepG2 transiently transfected with HBx were used as controls. (E) qPCR analysis of COX2, TNF-α, MTA1, and HBx mRNAs in HepG2 cells with or without MTA1 knockdown by siRNA-MTA1 after being transfected with control vector or vector expressing HBx. Control siRNA was used in indicated experiments. Expression levels of COX2, TNF-α, MTA1, and HBx were normalized with β-Actin. Inset: Western blot analysis for MTA1 in HepG2 cells after being co-transfected with siRNA-MTA1 and HBx or control vector. Vinculin was used as a control.