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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Anal Chem. 2013 Feb 26;85(6):3095–3103. doi: 10.1021/ac302676q

Figure 3.

Figure 3

Effect of buffer conductivity on the flow-gatedinjection. 200 V, 200 V and −4500 V were applied to R1, R5 (no capillary was connected) and R3, respectively. R2 was switched between “GND” (0.5 s) and “floating” (14.5 s) continuously, while R4 was disabled. R1 was filled with 3.4 μM ThioGlo-1 in 20 mM Tris-HCl (pH=7.50). (A) R2, R3, and R5 have 20 mM Tris-HCl (pH=7.50). (B) R2 and R3 as in (A), R5 has ACSF (pH=7.40). (C) R2/R3 has 20 mM Tris-HCl containing 60 mM NaCl (pH=7.50),R5 same as (B).