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. Author manuscript; available in PMC: 2013 Apr 9.
Published in final edited form as: J Immunol. 2009 Dec 15;183(12):8119–8127. doi: 10.4049/jimmunol.0901925

Figure 1. MMP-1 and MMP-3 induce macrophage expression of Cox-2.

Figure 1

[A] RAW264.7 macrophages or thioglycollate-elicted peritoneal macrophages were suspended in DMEM containing 10% FCS and dispersed into a 48-well plate (2×105 cells/well). Following adherence, cells were washed 3X with DPBS and media were replaced with DMEM-0.1% low endotoxin (LE) BSA alone (Ctrl) or DMEM-0.1% LE-BSA containing 50 nM MMP-1, MMP-2, MMP-3, MMP-7, MMP-9 or 10 ng/ml LPS. The next day cell lysates were prepared, and levels of Cox-2 and GAPDH were determined utilizing Western blot. [B] Macrophages were dispersed into a 6-well plate (2×106 cells/well). Following adherence, cells were washed. Media were replaced with DMEM-0.1% LE-BSA alone (Ctrl) or DMEM-0.1% LE-BSA containing 50 nM MMP-1, MMP-3 or 10 ng/ml LPS. After overnight incubation, total RNA was isolated, and mRNA levels for Cox-2 and actin were determined utilizing RT-PCR. The data are representative of three separate experiments.