Figure 2. Coordination of NPC and NE assembly at post-mitosis. (A) Scheme showing effects of ELYS/Nup107–160 complex on postmitotic NE/NPC assembly. In the presence of ELYS/Nup107–160 complex, scaffold Nups (Nup107–160 complex, Nup93–205 complex, also Pom121 and Ndc1), LBR and emerin, target to the noncore region of mitotic chromosomes soon after anaphase onset. During anaphase/telophase, A-type lamin binding proteins (represented by emerin) and A-type lamins accumulate at the core region of mitotic chromosomes. The core regions become the pore-free islands whereas noncore regions become the pore-rich region on the assembled, functional NE at early G1. In the absence of ELYS/Nup107–160 complex, neither scaffold Nups nor LBR can target mitotic chromosomes, although B-type and A-type lamins can localize to chromosomes in ananphase/telophase. Because absence of ELYS/Nup107–160 complex affects proper localization of the chromosomal passenger complex (CPC) that contains Aurora B kinase, the phosphorylation gradient is disrupted, which causes an aberrant distribution of core region proteins, including A-type lamins and their binding partners and also disturbs cytokinesis. RanGTP–gradient is established throughout cell cycle by chromosome bound RCC1. S-Nups, scaffold Nups; P-Nups, peripheral Nups; NPC, Nuclear Pore Complex. (B) Left: Photograph showing core region and noncore region in telophase (HeLa cell): red, LBR; green, emerin; blue, ELYS/Mel28. Right: Photographs of the nuclear surface of early G1 HeLa cells shows IF staining of emerin (upper picture), which accumulates at pore-free islands and IF staining of NPCs (lower picture), representing the pore-rich region. Pore-free islands originate from the mitotic core regions, whereas pore-rich regions derive from mitotic noncore regions as depicted in the scheme.