Figure 1. Selective loss of Nopp140 in Act5C > UAS-C4.2 larvae. Expression of RNAi targeting Nopp140 transcripts caused depletion of Nopp140 in most tissues as shown by DAPI staining and immuno-fluorescence microscopy using an antibody directed against a unique sequence within the carboxy terminal domain of Nopp140-RGG.⁶(A and B) Nucleoli in most small diploid imaginal disc cells from Act5C > UAS-C4.2 larvae lacked Nopp140-RGG. A few cells retained detectable Nopp140-RGG in their nucleoli (B), due presumably to insufficient RNAi expression in these particular cells. (C and D) Nucleoli in giant polyploid cells from Act5C > UAS-C4.2 larvae also lacked Nopp140-RGG. Polyploid cells lacking Nopp140 contained condensed chromatin.⁶(E and F) Nucleoli in most imaginal disc cells from wild type larvae stained with anti-Nopp140-RGG. (G and H) Nucleoli in polyploid midgut cells and the smaller imaginal island cells from wild type larvae stained well with the anti-Nopp140-RGG antibody. Scale bar: 25 µm for all images.