Figure 3. Loss of Nopp140 in imaginal wing discs of A9 > UAS-C4.2 larvae resulted in apoptosis. Phase contrast (A) and DAPI staining (B) of a wing disc isolated from an A9 > UAS-C4.2 larva. (C) The wing disc stained heavily with anti-Caspase 3 indicating apoptosis. (D and E) Higher magnification of panels B and C showed anti-Caspase 3 staining in the cytoplasm as expected. Cells with copious anti-Caspase 3 labeling showed condensed chromatin by DAPI staining (arrows). (F−H) Wing discs from control (w¹¹¹⁸) larvae were probed with anti-Caspase 3 antibody. They showed minimal background labeling.