Figure 5. Autophagy was prominent in larval polyploid cells depleted for Nopp140. (A and D)da > mCherry-ATG8 in a wild type background. Labeling remained diffuse throughout the cell. (B and E) As a positive control for autophagy, similar da > mCherry-ATG8a larvae were starved of amino acids. The accumulation of mCherry-ATG8a into autophagosomes and lysosomes (red speckling in the cytoplasm) indicated autophagy as expected due to starvation. (C and F) Well fed da > UAS-C4.2; mCherry-ATG8a larvae displayed similar accumulations of mCherry-ATG8a into cytoplasmic vesicles within their midgut cells indicating premature autophagy in response to the loss of Nopp140.