Figure 7. Pro-apoptotic Hid and activated JNK were both upregulated in Nopp140-depleted larvae. (A) An immuno-blot containing lysates from parental control larvae (lanes 1–4) and Act5C > Nopp140-RNAi larvae (lanes 5–8) was probed with rabbit anti-Hid.34 Hid at ~50 kDa was substantially increased in the Nopp140-depleted larvae as compared with parental larvae. Lane 1: homozygous UAS-C4.2; Lane 2: Act5C-GAL4/CyO; Lane 3: Act5C-GAL4/+; Lane 4: CyO-GFP/+, Lanes 5 and 7: UAS-C4.2/Act5C-GAL4; Lanes 6 and 8: UAS-C4/Act5C-GAL4. UAS-C4 is the original transgenic stock that contains UAS-C4.2 on the 2nd chromosome and UAS-C3 on the 3rd chromosome. (B) A companion blot with the same lysates was probed with rabbit monoclonal antibody 81E11 (Cell Signaling #4668) directed against phospho-JNK at 43 kDa. (C) Equal amounts of the same lysates as used in panels A and B were resolved on a Coomassie-stained SDS-gel. Phenoloxidase A3 (PO) is at 70 kDa.