Figure 4. Localization of lamins by indirect immunofluorescence in FTI-treated cells. BJ-5ta cells were treated with 2.5 μM L744,832 for 40 h prior to fixation as described in Materials and Methods. (A) Representative images of cells immunostained to localize LA/C, preLA and LB2. Bar = 10 μm. Arrows indicate donut shaped nuclei. (B) Quantification of the nuclear envelope/lamina (NE) signal intensity for each lamin. (C) Quantification of the nucleoplasmic intensity for each lamin. For the measurements in (B) and (C), the number of nuclei scanned for each lamin was: LA/C (13), LA/C FTI (17), preLA (15), preLA FTI (13), LB1 (14), LB1 FTI (13), LB2 (20) and LB2 FTI (14). Arrow indicates the minimum or maximum outliers.