Figure 1. D4 mRNA expression in Tg_Ped/pea-15 mice.

D4 mRNA expression was determined by quantitative Real Time RT-PCR analysis of total RNA isolated from liver (A), pancreas (B), and tibialis skeletal muscle tissue (C) of Tg_Ped/pea-15 mice at one week post Ad-D4 or Ad-GFP infection. GAPDH was used as housekeeping gene. mRNA levels in Ad-D4 treated Tg_Ped/pea-15 mice are relative expression units to those in Ad-GFP treated Tg_Ped/pea-15 mice used as control (mean ± SEM; n = 3). ***p<0.001 vs Ad-GFP treated Tg_Ped/pea-15.