Table I.
Primary antibodies, clones, dilution ratios and sources used for immunohistochemical staining.
| Antibodies | Clone | Dilution | Source |
|---|---|---|---|
| Estrogen receptor (ERa) | 6F11 | 1:100 | Neomarkers |
| Estrogen receptor (ERb) | SP1 | 1:100 | Neomarkers |
| Progesterone receptor (PRa) | PgR 312 | 1:200 | Novocastra |
| Progesterone receptor (PRb) | PgR 636 | 1:400 | Dako |
| HER2a | CB11 | 1:200 | Novocastra |
| HER2b | Rabbit polyclonal | 1:2,000 | Dako |
| Ki-67 | MIB-1 | 1:800 | Dako |
| CD44 | F10-44-2 | 1:40 | Novocastra |
| CD24 | SN3 | 1:50 | Neomarkers |
| CD133 | Rabbit polyclonal | 1:100 | Abcam |
| Cytokeratin 5 (CK5) | XM26 | 1:300 | Neomarkers |
| EGFR | EGFR-25 | 1:100 | Novocastra |
Detected using the
a
MACH 4 Universal HRP-Polymer and
b
Advance HRP detection systems.