A. Examples of electrophysiological recordings showing miniature (TTX resistant) GABAergic synaptic currents in DMH cholinergic neurons following control vs. overnight fasting conditions B and C. Summary plots show that fasting did not alter miniature GABAergic synaptic transmission (control: n = 21 neurons, Fasting: n = 15 neurons). D. Pooled data showing no difference in the frequency or amplitude of miniature IPSCS recorded in DMH cholinergic neurons (Filled circle, control; open circle, fasting). E. There was no change in the decay time constants of miniature IPSCs recorded in DMH cholinergic neurons. F. Sample recording traces showing miniature glutamatergic synaptic transmission (mEPSCs). G, H, I and J. Pooled data show that overnight fasting did not change the frequency, amplitude and decay time constant of miniature EPSCs (n = 31 neurons, Fasting: n = 24 neurons; Filled circle, control; open circle, fasting; control).