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. 2013 Apr 9;8(4):e61338. doi: 10.1371/journal.pone.0061338

Figure 1. Immunophenotyping (a) and gene-expression (b) of Eμ-ALL01 cells.

Figure 1

Immunophenotyping was performed with the antibodies listed on the axes. Cells were first gated within a live lymphoid gate on a scatter plot. The bottom panels are isotype (iso) or fluorescence minus one (FMO) controls. (b) RT-PCR was performed for the listed genes and electrophoresed through a 1% agarose gel and stained with Ethidium Bromide. No-template (NT) and Cd8, which is expressed usually in T cells but not B cells, are included as negative controls. MWT refers to the 1 Kb Plus DNA ladder (Invitrogen). The triangles point to the 1 Kb (top) and 100 bp fragments.