Figure 2. Progressive disease in mice after injection with Eμ-ALL01 tumor cells.
(a) Retro-orbital blood was collected from 5 mice on the days indicated after Eμ-ALL01 injection and analyzed serially over time with an ACT diff cell counter (Beckman Coulter, Brea, CA). White cell count (WBC), Hemoglobin (Hgb), and Platelets were measured and statistically analyzed with t-tests, which were non-significant for Day 0 and Day 14 means. Comparison of the Day 0 and Day 32 means by t-tests were all significant (p values are noted on graph). Error bars are the standard error of the mean (SEM). The mice were sacrificed 4 weeks after injection with Eμ-ALL01 because of clinical deterioration and retro-orbital blood, bone marrow, and spleen were harvested for anatomical (b) and cellular (c) analyses, with representative images displayed. (b) Blood and tissue were used to prepare peripheral smears (left-panel) and H&E stained slides of sections of the bone marrow (middle-panel) and spleen (right-panel). (c) Single-cell suspensions were prepared from bone marrow (BM) and spleen (SP) and then incubated with antibodies specific for B220 and IgM. The panels on the top are cells isolated from disease-free, wild-type C57BL/6 mice (B6) and the panels on the bottom were injected with the Eμ-ALL01 cells. Cells displayed have been gated on live lymphoid cells.