Table 1. Summary of results for the number of features found using MFE, an “untargeted” feature finding algorithm.
Condition | pH2 | pH7 | pH9 | pH2 | pH7 | pH9 | pH2 | pH7 | pH9 |
ESI− | ESI− | ESI− | ESI+ | ESI+ | ESI+ | APCI + | APCI + | APCI + | |
NRBC | 1433 | 915 | 1267 | 2029 | 1401 | 2626 | 1128 | 463 | 500 |
IRBC | 1327 | 1151 | 1232 | 2042 | 1542 | 2661 | 665 | 460 | 652 |
*Rel Freq. 75% | 1660 | 1282 (245) | 1412 (273) | 2175 (602) | 1838 (602) | 2822 (650) | 916 | 502 | 679 |
*Rel Freq. 100% | 1259 | 917 (124) | 1086 (178) | 1550 (425) | 1247 (556) | 2086 (378) | 486 | 342 | 483 |
CV<100% | 781 | 547 (106) | 649 (161) | 936 (376) | 662 (387) | 1083 (350) | 284 | 235 | 271 |
The numbers in parentheses () denote the number of features found by LC/MS using ANP chromatography. Data was not collected for APCI+mode and for pH 2 ANP/ESI−.
The % relative frequency (Rel Freq.) corresponds to the number of features found in 75% or in 100% of all biological sample replicates, and in at least one or more conditions. Only features with a coefficient of variation less than 100% (CV<100%) were used for subsequent significance testing.