Table 1.

Volumes for preclearing the chromatin

	Chromatin	Lysis Buffer	Dilution Buffer	Preblocked A/G beads
Sonicated chromatin from plate	10 µl (~4 µg)	90 µl	900 µl	50 µl
Chromatin from 100 K cells	100 µl (~1 µg)	0 µl	900 µl	50 µl