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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Anal Chem. 2012 Dec 28;85(2):1065–1072. doi: 10.1021/ac3028273

Figure 3.

Figure 3

Estimates of LQQC*PFEDHVK adducts recovered from trypsin/chymotrypsin digestion of m-CPBA-oxidized or nonoxidized HONH-PhIP-modified SA, without or with denaturation. Adduct formation was estimated by UPLC-ESI/MS2, employing LQQC*PF (C-[SO2]-[2H5]-PhIP) sulfonamide and LQQC*PF (C-[S=O]-[2H5]-PhIP) sulfinamide as internal standards. The ionization efficiencies of the mixed cleavage peptide adducts LQQC*PFEDHVK were assumed to be comparable to the ionization efficiencies of the internal standards of [2H5]-PhIP-modified LQQC*PF. Total PhIP bound to SA was determined by UV measurement at 320 nm.14