Table 1. Amino acid sequences and characterization features of the synthetic peptides.
| Peptide | Sequencea | RetentionTime (min)b | Mr(g mol−1) | Purity (%) |
| Ctx(Ile21)-Ha | GWLDVAKKIGKAAFNVAKNFI | 18.270 | 2,289.7 | 96 |
| [TOAC0]Ctx(Ile21)-Ha | OGWLDVAKKIGKAAFNVAKNFI | 18.611 | 2,486.0 | 97 |
| [TOAC2]Ctx(Ile21)-Ha | GOLDVAKKIGKAAFNVAKNFI | 18.494 | 2,299.8 | 98 |
| [TOAC13]Ctx(Ile21)-Ha | GWLDVAKKIGKAOFNVAKNFI | 21.038 | 2,414.9 | 96 |
a
The letter (O) represents the paramagnetic amino acid TOAC.
b
Values obtained from analytical RP-HPLC with C18 column and program: 5–95 % B in 30 min. Solvent A: 0.045% TFA: H2O and B: 0.036% TFA: ACN. The peptides were eluted at 1.0 mL min−1 of flow rate and detected at 220 nm.