Table 3.
Multiple laccase isoenzymes produced by C. cinerea strains during cultivation at 25 °C in mKjalke medium (plus 0.1 mM CuSO4) as identified from Mascot analysis (for detailed results see Supplementary Table S1)
| Laccase banda | Coprinopsis cinerea monokaryonsb | ||||||
|---|---|---|---|---|---|---|---|
| 218 | 306 | AT8 | FA2222 | H5 | JV6 | PG78 | |
| 1 | ± | + | + | ± | ± | – | – |
| 2 | + | (Lcc5) | – | + | Lcc1/5 | + | – |
| 3 | – | – | – | – | – | + | – |
| 4 | – | – | – | – | – | – | + |
| 5 | – | – | Lcc9 | + | – | – | ± |
| 6 | – | Lcc2 | ± | – | – | (Lcc2) | – |
| 6/7 | – | Lcc9/10 | – | – | – | – | – |
| 7 | + | Lcc10 | Lcc10 | – | – | – | – |
| 8 | Lcc1 | Lcc1 | Lcc1 | Lcc1 | Lcc1 | Lcc1 | Lcc1 |
| 9 | – | – | Lcc1 | – | – | – | + |
| 10 | Lcc5 | Lcc5 | Lcc5 | Lcc5 | Lcc1/(5) | Lcc5 | Lcc5 |
aThe number of a band refers to a respective laccase band in Fig. 4
b+ and ± indicate presence of bands in Fig. 4 of minor or faint staining for which no laccase could be identified. – means that no band was detected. Italizised value of cells with names of identified enzymes correspond to strong bands in Fig. 4, remaining cells with names of identified enzymes to faint bands. Laccase names in brackets indicate non-significant hits (only 1 peptide detected with an ion score >43)