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Constructs were generated by progressively cloning 5′-truncated CD274 promoter fragments into the pGL3-basic luciferase vector. Negative numbers denote bp distances from the transcriptional start site (panel A). U937 cells were electroporation cotransfected with these reporter constructs and pRL-TK to control for transfection efficiency. Cells were either unstimulated (panel B) or stimulated with LPS (panel C) for 24 h.
