Table 1.
Primer name | Orientation | Sequence (5′ to 3′) | Target |
---|---|---|---|
Os07g49370 F | Sense | CACCATGGCGTCGGCAGGTGGCTGCAAG | Os07g49370 |
Os07g49370 R | Antisense | CTAGAGCGTAGTTTGGATGCG | Os07g49370 |
Os01g48440 F | Sense | CACCATGTCCCGAAGGAATGCCGGGGCA | Os01g48440 |
Os01g48440 R | Antisense | TTATGTTATTGGCACAACAGCATC | Os01g48440 |
Os06g47340 F | Sense | CACCATGATGAAGTCGCTGCTGCCG | Os06g47340 |
Os06g47340 R | Antisense | TCAGTTCTCCTTCCGCTTTGTGGT | Os06g47340 |
Os07g49370 qPCR F | Sense | CTCCGGAGACGTTAATGGAAGT | qRT PCR amplicon Os07g49370 |
Os07g49370 qPCR R | Antisense | CTGCACGAACTTCACTGATTCC | qRT PCR amplicon Os07g49370 |
Os01g48440 qPCR F | Sense | GTATAGTGCATTTCGCTGATGAAG | qRT PCR amplicon Os01g48440 |
Os01g48440 qPCR R | Antisense | TTCTAGAACCACTCTGTACTTTGTCC | qRT PCR amplicon Os01g48440 |
Os06g47340 qPCR F | Sense | GTCACGCAACCGAGAATCGTAT | qRT PCR amplicon Os06g47340 |
Os06g47340 qPCR R | Antisense | AGCTATGAACATTGCTGTCATCC | qRT PCR amplicon Os06g47340 |
UBQ 10 F | Sense | GGCCTTGTATAATCCCTGATGAATAAG | qRT PCR reference gene |
UBQ 10 R | Antisense | AAAGAGATAACAGGAACGGAAACATAGT | qRT PCR reference gene |
IRX9F | Sense | GCTGGTAAGGCCTCATTTTTC | Genotyping irx9, Salk_058238 |
IRX9R | Antisense | AACTTACCAACCCACCCATTC | Genotyping irx9, Salk_058238 |
IRX14F | Sense | AACGACACGTGTACCTCCTTG | Genotyping irx14, Salk_ 038212 |
IRX14R | Antisense | AACATCACAATCCCATCAAGC | Genotyping irx14, Salk_ 038212 |
LBa1 | Sense | TGGTTCACGTAGTGGGCCATCG | Left border primer of SALK lines |