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. 2013 Apr 10;4:83. doi: 10.3389/fpls.2013.00083

Table 1.

List of primers used for gene cloning and quantitative PCR.

Primer name Orientation Sequence (5′ to 3′) Target
Os07g49370 F Sense CACCATGGCGTCGGCAGGTGGCTGCAAG Os07g49370
Os07g49370 R Antisense CTAGAGCGTAGTTTGGATGCG Os07g49370
Os01g48440 F Sense CACCATGTCCCGAAGGAATGCCGGGGCA Os01g48440
Os01g48440 R Antisense TTATGTTATTGGCACAACAGCATC Os01g48440
Os06g47340 F Sense CACCATGATGAAGTCGCTGCTGCCG Os06g47340
Os06g47340 R Antisense TCAGTTCTCCTTCCGCTTTGTGGT Os06g47340
Os07g49370 qPCR F Sense CTCCGGAGACGTTAATGGAAGT qRT PCR amplicon Os07g49370
Os07g49370 qPCR R Antisense CTGCACGAACTTCACTGATTCC qRT PCR amplicon Os07g49370
Os01g48440 qPCR F Sense GTATAGTGCATTTCGCTGATGAAG qRT PCR amplicon Os01g48440
Os01g48440 qPCR R Antisense TTCTAGAACCACTCTGTACTTTGTCC qRT PCR amplicon Os01g48440
Os06g47340 qPCR F Sense GTCACGCAACCGAGAATCGTAT qRT PCR amplicon Os06g47340
Os06g47340 qPCR R Antisense AGCTATGAACATTGCTGTCATCC qRT PCR amplicon Os06g47340
UBQ 10 F Sense GGCCTTGTATAATCCCTGATGAATAAG qRT PCR reference gene
UBQ 10 R Antisense AAAGAGATAACAGGAACGGAAACATAGT qRT PCR reference gene
IRX9F Sense GCTGGTAAGGCCTCATTTTTC  Genotyping irx9, Salk_058238
IRX9R Antisense AACTTACCAACCCACCCATTC Genotyping irx9, Salk_058238
IRX14F Sense AACGACACGTGTACCTCCTTG Genotyping irx14, Salk_ 038212
IRX14R Antisense AACATCACAATCCCATCAAGC Genotyping irx14, Salk_ 038212
LBa1 Sense TGGTTCACGTAGTGGGCCATCG Left border primer of SALK lines