Skip to main content
. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Eur J Neurosci. 2012 Jan;35(2):300–308. doi: 10.1111/j.1460-9568.2011.07956.x

Fig. 3.

Fig. 3

Dopamine and cAMP-regulated phosphoprotein (DARPP-32) expression is reduced in the striatum from regulator of CaM signaling (RCS) knockout (KO) mice. Proteins from striatal (STR), amygdala (AMY) and hippocampal (HIP) extracts were analysed by SDS–PAGE and immunoblotting using RCS, DARPP-32, striatal enriched phosphatase 46 kDA (STEP-46) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies. Representative immunoblots are shown in (A), and quantitation is shown in (B) and (C). Expression levels of DARPP-32 and STEP-46 were normalized to that of GAPDH. Error bars indicate SEM, n = 5–7 per group. DARPP-32 levels were decreased in RCS KO striatal homogenates by 38% compared with wild-type (WT) mice (F1,11 = 13.780, P = 0.004). Levels of STEP-46 were unchanged in striatal homogenates (F1,11 = 0.751, P = 0.40) from WT and RCS KO mice.