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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Adv Mater. 2011 Sep 28;23(42):4908–4912. doi: 10.1002/adma.201102677

Figure 3. Examination of effect of T30 crosslinking on ultrasound-induced microbubble dynamics.

Figure 3

(a) Comparison of percentage of oscillating microbubbles vs. incident pressure for uncrosslinked (green) and crosslinked (red) microbubbles. (b,c) Bright field and green fluorescence micrographs of DPPC:DPPA:DSPE-PAA-A15 microbubbles with FAM-T30 added. The green fluorescence of the crosslinking strand only appears on the microbubble shell. (d) B-mode and (e) contrast-enhanced ultrasound images obtained simultaneously for uncrosslinked (left) and crosslinked (right) microbubbles in a phantom. The bright spots at the top and bottom of the B-mode image are caused by elastic reflection of ultrasound from the front and back walls of the phantom, respectively. The outline of the round phantom is observed as well. The contrast-enhanced image (e) shows that the uncrosslinked bubbles (left) are significantly brighter than crosslinked bubbles (right). A comparison of these images reveals that the contrast-signal overlays with the interior of the phantom, demonstrating the selectivity of microbubble imaging.