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. 2013 Apr 10;3:1638. doi: 10.1038/srep01638

Figure 3.

Figure 3

(a) Residence time as a function of applied voltage for three SAP97 PDZ2 domains with varying stabilities (wild type (WT) SAP97 PDZ2 > V388A > L322A).(b) Excluded volumes as a function of voltage for the same three SAP97 PDZ2 domain variants. The domains were diluted in 2 M KCl and 10 mM potassium phosphate buffer (pH 7). Data was collected with pores 15 ± 2 nm in diameter for the I342W domain (n200mV = 202, n400mV = 1005, n600mV = 1474, n800mV = 1006), the V388A domain (n200mV = 1719, n300mV = 703, n600mV = 768, n800mV = 1493), and the L322A domain (n200mV = 233, n400mV = 674, n600mV = 607, n800mV = 550).