| % of male DNA in Female Background | Volume (μl) of 4ng/μl male DNA | Volume (μl) of 4ng/μl female DNA | Total volume(μl) |
| 0.2 | 1 | 499 | 500 |
| 0.5 | 1 | 199 | 200 |
| 2.5 | 5 | 195 | 200 |
| 12.5 | 25 | 175 | 200 |
| 50 | 100 | 100 | 200 |
| 87.5 | 175 | 25 | 200 |
| 100 | 200 | 0 | 200 |
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