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. 2013 Feb 28;(72):50232. doi: 10.3791/50232
DAY PROCEDURE
1 DM = STZ Injection (350 mg/dl), Control = saline injection
3 DM = STZ Injection (350 mg/dl), Control = saline injection
5 DM = STZ Injection (350 mg/dl), Control = saline injection
12 DM = STZ Injection (350 mg/dl), Control = saline injection
19 DM = STZ Injection (350 mg/dl), Control = saline injection
21 Either perform assay of interest for DM fish or proceed to make MM groups by removal of STZ pressure.
51 Amputate Fins 30 days following the last STZ injection of controls, DM and STZ groups in order to generate MM tissue.
81 Re-amputate fins of all groups in the tissue that was grown between day 51 and 81 to perform regeneration study. Alternatively treat fish/tissue with the assay of interest.