| DAY | PROCEDURE |
| 1 | DM = STZ Injection (350 mg/dl), Control = saline injection |
| 3 | DM = STZ Injection (350 mg/dl), Control = saline injection |
| 5 | DM = STZ Injection (350 mg/dl), Control = saline injection |
| 12 | DM = STZ Injection (350 mg/dl), Control = saline injection |
| 19 | DM = STZ Injection (350 mg/dl), Control = saline injection |
| 21 | Either perform assay of interest for DM fish or proceed to make MM groups by removal of STZ pressure. |
| 51 | Amputate Fins 30 days following the last STZ injection of controls, DM and STZ groups in order to generate MM tissue. |
| 81 | Re-amputate fins of all groups in the tissue that was grown between day 51 and 81 to perform regeneration study. Alternatively treat fish/tissue with the assay of interest. |