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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 18;190(8):4263–4273. doi: 10.4049/jimmunol.1200732

Figure 7.

Figure 7

Expression of SLC11A1 rendered MOLT14 human γδ T cells more prone to activation. MOLT14 cells were electroporated with a plasmid expressing SLC11A1-GFP and stimulated for 24 hours with various agonists. A. Cells expressing SLC11A1-GFP expressed significantly more CD69, both resting and following activation with ConA/IL-2 (100 ng/ml). B. SLC11A1-GFP+ cells expressed significantly more CD69 in response to conditioned media. Media from unstimulated and LPS-stimulated human PBMCs (from n=5 donors) collected after 24 hours were used to stimulate SLC11A1-GFP transfected MOLT14 cells, resulting in increased expression of CD69, in the LPS-conditioned media in particular. C. SLC11A1-GFP+ cells expressed significantly more IFN-γ in response to both types of conditioned media. These results represent pooled data from 3 independent experiments. Error bars represent standard deviation, Students t test ***p<0.001, **p<0.01, *p<0.05.