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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 18;190(8):4263–4273. doi: 10.4049/jimmunol.1200732

Figure 8.

Figure 8

Non-adherent splenocytes from wild type mice express greater IFN-γ than do cells from congenic SLC11A1-deficient mice. Adherent cells from 129/Sv and 129/Sv (SLC11A1−/−) mice were infected with 2 doses of Salmonella for 8 hours. Supernatant fluids from these cultures were sterile filtered and applied to non-adherent cells from the 2 mouse strains. Forty-eight hours later, IFN-γ expression was assessed by ELISA. A. Non-adherent cells from wild type mice expressed more IFN-γ. The difference was most significant after infection of adherent cells with the low dose of Salmonella. B. Stimulation with IL-12 or ConA/IL-2 did not replicate the differences between the non-adherent cell types. Representative data is shown with significance based on triplicate wells, experiment was performed three times, Students t test ***p<0.001, **p<0.01, *p<0.05.