Skip to main content

View full-text article in PMC

. 2013 Apr 9;2:e00426. doi: 10.7554/eLife.00426

Table 3.

Apparent binding affinity (Kd) and maximal binding (Bmax) for CLOCK:BMAL1 and USF at four different E-boxes in Figure 7

DOI: http://dx.doi.org/10.7554/eLife.00426.013

	CLOCK:BMAL1	CLOCK^Δ19:BMAL1	USF1 WT	USF1 Clock^Δ19/Clock^Δ19
Dbp E_P-box
Bmax	0.97 ± 0.02	1.25 ± 0.05 *	0.83 ± 0.06	0.87 ± 0.04
Kd	2.01 ± 0.22^*	32.94 ± 3.92	42.8 ± 9.30	49.56 ± 6.20
Dbp E_I2-box
Bmax	0.79 ± 0.02^*	2.38 ± 0.05	2.25 ± 0.05	2.10 ± 0.05
Kd	0.71 ± 0.10^*	6.18 ± 0.54	6.10 ± 0.52	9.64 ± 0.96
Per2 E′-box
Bmax	0.26 ± 0.02^*	0.43 ± 0.02	0.36 ± 0.03	0.41 ± 0.02
Kd	2.00 ± 0.64^*	8.05 ± 1.57	17.81 ± 5.08	16.71 ± 2.46
Per1 E_P1-box
Bmax	0.74 ± 0.12^*	7.38 ± 1.18	3.06 ± 0.64	7.96 ± 2.01
Kd	16.55 ± 9.49	43.69 ± 19.40	33.36 ± 20.99	58.62 ± 37.82

Values are the mean + SEM derived from four-parameter nonlinear curve-fitting.

^*

indicates that the Bmax or Kd is significantly different from other groups, p<0.0001.