Figure 6.

Anti-CD40 results in early development of TEC H/P in IFN-γ−/− NOD.H-2h4 mice. A. All mice were given NaI in their drinking water. At various times after injection of anti-CD40 or isotype control, thyroids were removed and scored for severity of thyrocyte proliferation. * p < 0.01, Mann Whitney. Error bars are ± SEM, and represent 4 experiments. For isotype, n = 10; anti-CD40, 1 wk, n = 15; 4 wk, n = 15; 8 wk, n = 18, and isotype 6–7 mo, n = 10. B. CD40 expression by thyrocytes of IFN-γ−/− NOD.H-2h4 mice with TEC H/P. Images are representative of 2 experiments, n = 6. 100× magnification bar is 0.01 mm, 400× magnification bar is 0.05 mm. C. Splenocytes from IFN-γ−/− NOD.H-2h4 mice with severe TEC H/P were cultured and transferred to IFN-γ−/− SCID NOD.H-2h4 mice as described in Methods. CD40 expression was examined 4 and 8 wk later in mice with varying TEC H/P severity scores. Images are representative of 2 experiments, n= 6. D. CD40 protein expression in thyroids of IFN-γ−/− mice with 0+ or 5+ TEC H/P severity scores or IFN-γ−/− mice given anti-CD40 1 wk earlier. Data was quantified by comparing the ratio of CD40 to actin as in Figure 3. Results are representative of 2 experiments, n=6. Error bars are ± SEM; * p<0.05, Student's t test.