Table I.
Effects of anti-CD40 on thyroids of different strains of mice
| Strain | 6–10 daysa | 3–8 wka | Serum T4 b |
|---|---|---|---|
| IFN-γ−/− NOD.H-2h4 SCID | 4–5+ (n = 25 ) | 4–5+ (n=13) | Low |
| IFN-γ−/− NOD.H-2h4 | 4–5+ (n=18) | 4–5+ (n=28) | Low |
| CD4−/− IFN-γ−/− NOD.H-2h4 | 4–5+ (n=20) | 4–5+(n=16) | Low |
| B cell −/− IFN-γ−/− NOD.H-2h4 | 4–5+ (n=5) | 4–5+ (n=9) | NDc |
| WT NOD.H-2h4 | 1–4+ (n=10) | 0–3+d (n=11) | Normal |
| CD40−/− NOD.H-2h4 | 0 (n=8) | ND | Normal |
| IFN-γ−/− NOD | 4–5+ (n=8) | 4–5+ (n=6) | Low |
| WT NOD | 0+−4+ (n=17) | 0+ (n=8) | Normal |
| Fc null NOD | 0+−4+ (n=14) | ND | ND |
| NOD. SCID | 4+ (n=9) | 4+ (n= 7) | Normal |
| IFN-γ−/− DBA/1 | 0 (n = 11) | 0 (n=6) | Normal |
| WT DBA/1 | 0 (n=3) | 0 (n=6) | Normal |
| CBA/J | 0 (n = 5) | ND | Normal |
| B6 Rag−/− | 0 (n = 3) | 0 (n = 3) | ND |
| Balb/c Rag−/− | 0 (n = 3) | ND | ND |
The indicated strains of mice were given 200 μg anti-CD40 and thyroids were removed after 6–10 days or 3–8 wk. TEC H/P severity scores are assigned as described in Methods. The range shown for each strain does not include an occasional mouse in the larger groups that failed to respond to anti-CD40, presumably because of an ineffective i.p. injection. Numbers of mice in each group are indicated in parentheses.
Serum T4 levels 6–10 days and 3–8 wk after anti-CD40. Normal serum T4 is defined as ≥ 3 μg/dL and mice with low serum T4 had T4 levels of ≤3 μg/dL. Mice with 5+ severity scores generally have low serum T4 levels whereas mice with 4+ or lower severity scores always have normal serum T4 levels.
ND = not determined.
WT NOD.H-2h4 mice given either isotype control or anti-CD40 had SAT but did not have TEC H/P 6–8 wk after receiving NaI in their water.