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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 11;190(8):4324–4336. doi: 10.4049/jimmunol.1202045

FIGURE 3.

FIGURE 3

Increased expression of inflammatory markers on cutaneous DCs following ex vivo stimulation with BzATP. Skin explants were injected with 350 µM of BzATP or PBS (No Tx). 24 and 72 h following injections skin sections were immunofluorescently labeled with Langerin, HLA-DR, and (A) DC-LAMP, (D) CD206, or (G) iNOS. Dotted line represents epidermal-dermal junction and ^ indicates representative Langerin+ cells that are expressing DC-LAMP, CD206, or iNOS. Merged panels include all three stains and DAPI nuclear counter-stain. (E) Characterization of CD206+ cells 72 h following BzATP treatment. CD206-red and CD163-green. * indicates representative DCs (CD206+CD163−) and ^ indicates representative macrophages (CD206+CD163+). 40× original magnification, bar= 20µm. (B, C, F, H) Quantitation of cells positive for HLA-DR, DC-LAMP, CD206, or iNOS. Bars indicate the mean ± SD of 11–15 replicates. Asterisk indicates a significant difference, p< 0.05.