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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 11;190(8):4324–4336. doi: 10.4049/jimmunol.1202045

FIGURE 5.

FIGURE 5

Activation of smiDCs via P2X7R stimulates increased HMGB1 secretion and RAGE expression. SmiDCs were stimulated overnight with BzATP and/or KN-62. (A) Following overnight culture, supernatants were collected and assessed for the presence of HMGB1 by ELISA. Data expressed as mean ± SD of replicates. Asterisk indicates a significant difference between designated groups, p < 0.05. Data are representative of four independent experiments. (B) SmiDCs were collected and examined by flow cytometry for the surface expression of RAGE, an HMGB1 receptor,(filled histogram represents control antibody, dashed lined represents No Tx, grey line represents KN-62, and black line represents BzATP treatments), and (C) RAGE expression on Langerin+ cells, numbers represent the percent positive within the respective quadrant. Flow cytometry data is one representative of four independent subjects with similar results.